Product Information

Contents: Biotin anti-mouse CCR5 (CD195)
Catalog Number: 13-1951
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: HM-CCR5 (7A4)
Isotype: Armenian Hamster IgG

Staining of FC-blocked (anti-CD16/32, cat. 14-0161) BALB/c peritoneal-exudate cells with FITC anti-mouse/human CD11b (M1/70) (cat. 11-0112) and 0.25 μg of Biotin Armenian Hamster IgG Iso Cntrl (cat. 13-4888) (left) or 0.25 μg of Biotin anti-mouse CCR5 (HM-CCR5) (right) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1951	Affinity Purified anti-mouse CCR5 (CD195)	N/A	N/A	FC
13-1951	Biotin anti-mouse CCR5 (CD195)	N/A	N/A	FC
12-1951	Phycoerythrin (PE) anti-mouse CCR5 (CD195)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

CCR5 is the major co-receptor for macrophage-tropic isolates of HIV-1. CCR5, also known as CD195, is a seven transmembrane chemokine receptor capable of binding RANTES, MIP-1α and MIP-1β. Knockout mice show a decreased recruitment of macrophages and increased susceptibility to infection. Protein and mRNA has been found in macrophages from thioglycolate-treated and activated T cells.

The HM-CCR5(7A4) monoclonal antibody binds the N terminal extracellular domain of mouse CCR5.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HM-CCR5 (7A4) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HM-CCR5 (7A4) antibody has been tested by flow cytometric analysis of mouse thioglycolate elicited peritoneal cells (PECS). This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Iwasaki M, Mukai T, Nakajima C, Yang YF, Gao P, Yamaguchi N, Tomura M, Fujiwara H, Hamaoka T. 2001. A mandatory role for STAT4 in IL-12 induction of mouse T cell CCR5. J. Immunol. 167(12): 6877-83.

Meyer A, Coyle AJ, Proudfoot AE, Wells TN, Power CA. Cloning and characterization of a novel murine macrophage inflammatory protein-1 alpha receptor. J Biol Chem. 1996 Jun 14;271(24):14445-51.

Zhou Y, Kurihara T, Ryseck RP, Yang Y, Ryan C, Loy J, Warr G, Bravo R. Impaired macrophage function and enhanced T cell-dependent immune response in mice lacking CCR5, the mouse homologue of the major HIV-1 coreceptor. J Immunol. 1998 Apr 15;160(8):4018-25.

Related Products

Cat. 13-1957  Biotin anti-human CCR5 (CD195) (clone eBioT21/8 (T21/8))
Cat. 13-1958  Biotin anti-human CCR5 (CD195) (clone R22/7)
Cat. 13-1959  Biotin anti-human CCR5 (CD195) (clone V14/2)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4888  Biotin Armenian Hamster IgG Isotype Control (clone eBio299Arm)