Product Information

Contents: Biotin anti-human CD152 (CTLA-4)
Catalog Number: 13-1529
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 14D3
Isotype: Mouse IgG2a
HLDA No.: N/A

Staining of 3-day PHA activated normal human peripheral blood mononuclear cells with .03 μg of Biotin Mouse IgG2a, K Iso Cntrl (cat. 13-4724) (open histogram) or .03 μg of Biotin anti-human CD152 (14D3) (colored histogram) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1529	Affinity Purified anti-human CD152 (CTLA-4, CTLA4)	N/A	N/A	FA FC IH/F
16-1529	Functional Grade* Purified anti-human CD152 (CTLA-4, CTLA4)	N/A	N/A	FA FC
13-1529	Biotin anti-human CD152 (CTLA-4, CTLA4)	N/A	N/A	FC
12-1529	PE anti-human CD152 (CTLA-4, CTLA4)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 14D3 monoclonal antibody reacts with human CD152, also known as cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells (activated B cells may also express CTLA-4) and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in intracellular localization of a large proportion of newly synthesized CTLA-4 in response to TCR signaling.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 14D3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 14D3 antibody has been tested by flow cytometric analysis of PHA-stimulated human PBMCs. This can be used at less than or equal to 0.06 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.

References

Vandenborre K, et al. American Journal of Pathology (1998) 152: 963-73.
Vandenborre K, et al. Immunology (1999) 98: 1-10.
T.L. Walunas, et al. Immunity (1994) 1: 405-413.
N.J. Karandikar, et al. Journal of Experimental Medicine (1996) 184: 783-788.

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4724  Biotin Mouse IgG2a, K Isotype Control