Product Information

Contents: Biotin anti-mouse CD152 (CTLA-4)
Catalog Number: 13-1522
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: UC10-4B9
Isotype: Armenian Hamster IgG

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1522	Affinity Purified anti-mouse CD152 (CTLA-4, CTLA4)	N/A	N/A	FC IP
13-1522	Biotin anti-mouse CD152 (CTLA-4, CTLA4)	N/A	N/A	FC
12-1522	PE anti-mouse CD152 (CTLA-4, CTLA4)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The UC10-4B9 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells at low level and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The UC10-4B9 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The UC10-4B9 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.

References

Walunas, T. L., D. J. Lenschow, et al. 1994. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1(5): 405-13.
June, C.H., J.A. Bluestone, L.M. Nadler and C.B. Thompson (1994) The B7 and CD28 receptor families. Immunol Today 15: 231-331.
Krummel, M. F. and J. P. Allison (1995). "CD28 and CTLA-4 have opposing effects on the response of T cells to stimulation." J Exp Med 182(2): 459-65.

Related Products

Cat. 12-1521  PE anti-mouse CD152 (CTLA-4, CTLA4) (clone 9H10)
Cat. 14-1521  Affinity Purified anti-mouse CD152 (CTLA-4, CTLA4) (clone 9H10)
Cat. 16-1521  Functional Grade Purified anti-mouse CD152 (CTLA-4, CTLA4) (clone 9H10)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4888  Biotin Armenian Hamster IgG Isotype Control (clone eBio299Arm)