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Mouse Cellular Antigens Chart

Contents: Biotin anti-mouse CD150 (SLAM, IPO-3) Catalog Number: 13-1501 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 4°C. DO NOT FREEZE. Clone: 9D1 Isotype: Rat IgG1 Prices for This Product* Cat. No. Size Price Qty Action 13-1501-80 25 ug $105 13-1501-82 100 ug $275 *International customers: Please contact your distributor for region specific pricing.

Staining of C57Bl/6 splenocytes with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and 0.25 μg of Biotin Rat IgG1 Iso Cntrl (cat. 13-4301) (left) or 0.25 μg of Biotin anti-mouse CD150 (9D1) (right) followed by Streptavidin-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-1501	Fluorescein isothiocyanate (FITC) anti-mouse CD150 (SLAM, IPO-3)	488	518	FC
12-1501	Phycoerythrin (PE) anti-mouse CD150 (SLAM, IPO-3)	488	575	FC
17-1501	Allophycocyanin (APC) anti-mouse CD150 (SLAM, IPO-3)	633	660	FC
14-1501	Affinity Purified anti-mouse CD150 (SLAM, IPO-3)	N/A	N/A	FA  FC
16-1501	Functional Grade* Purified anti-mouse CD150 (SLAM, IPO-3)	N/A	N/A	FA  FC
13-1501	Biotin anti-mouse CD150 (SLAM, IPO-3)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.   Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The 9D1 monoclonal antibody reacts with mouse CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T (especially TH1) and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response. Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell. 9D1 is reported to be an activating antibody.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. This 9D1 antibody has been reported for use in flow cytometric analysis.

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Applications Tested

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This 9D1 antibody has been tested by flow cytometric analysis of mouse thymic and splenic cell suspensions. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Howie D, Okamoto S, Rietdijk S, Clarke K, Wang N, Gullo C, Bruggeman JP, Manning S, Coyle AJ, Greenfield E, Kuchroo V, Terhorst C. 2002. The role of SAP in murine CD150 (SLAM)-mediated T-cell proliferation and interferon gamma production. Blood. 100(8): 2899-907. (9D1, FC, FA, PubMed)

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Related Products

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Cat. 11-0481    Fluorescein isothiocyanate (FITC) anti-mouse CD48 (BCM1) (clone HM48-1)
Cat. 13-4301    Biotin Rat IgG1 Isotype Control
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)