Biotin anti-human CD144 (VE-Cadherin) antibody: biotin conjugate

Contents: Biotin anti-human CD144 (VE-Cadherin)
Catalog Number: 13-1449
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 16B1
Isotype: Mouse IgG1

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
13-1449-80	25 ug	$95
13-1449-82	100 ug	$220

*International customers: Please contact your distributor for region specific pricing.

Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.25 μg of Biotin Mouse IgG1, K Iso Cntrl (cat. 13-4714) (open histogram) or 0.125 μg of Biotin 16B1 (colored histogram) followed by Streptavidin-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-1449	Phycoerythrin (PE) anti-human CD144 (VE-Cadherin)	488	575	FC
17-1449	Allophycocyanin (APC) anti-human CD144 (VE-Cadherin)	633	660	FC
53-1449	Alexa Fluor® 488 anti-human CD144 (VE-Cadherin)	488	519	FC
56-1449	Alexa Fluor® 700 anti-human CD144 (VE-Cadherin)	633	723	FC
14-1449	Affinity Purified anti-human CD144 (VE-Cadherin)	N/A	N/A	FC WB
13-1449	Biotin anti-human CD144 (VE-Cadherin)	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 16B1 antibody has been reported for use in flow cytometric analysis, and immunocytochemistry.

Applications Tested

This 16B1 antibody has been tested by flow cytometric analysis of Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Stem Cells. 2007 Feb;25(2):490-9. (16B1, Immunocytofluorescence, PubMed)

Suzuki S, Sano K, and Tanihara H. 1991. Diversity of the cadherin family: evidence for eight new cadherins in nervous tissue. Cell Regul 2: 261-270.

Breviario F, Caveda L, Corada M, Martin-Padura I, Navarro P, Golay J, Introna M, Gulino D, Lampugnani MG, and Dejana E. 1995. Functional properties of human vascular endothelial cadherin (7B4/Cadherin-5), an endothelium-specific cadherin. Arterioscler Thromb Vasc Biol 15: 1229-1239.

Vincent PA, Xiao K, Buckley KM, and Kowalczyk AP. 2004. VE-Cadherin: adhesion at arm's length. Am J Physiol Cell Physiol 286: 987-997.

Related Products

Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)