Contents: Biotin anti-mouse CD135 (Flk-2/Flt-3, Ly-72)
Catalog Number: 13-1351
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: A2F10
Isotype: Rat IgG2a, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Two-color surface staining of mouse bone marrow with PE mouse IgG2a isotype control (left) or PE anti-mouse CD135 (A2F10) (right) and FITC CD45R/B220 (RA3-6B2). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-1351 |
Affinity Purified anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72) |
N/A |
N/A |
FA
FC
IP
|
| 16-1351 |
Functional Grade* Purified anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72) |
N/A |
N/A |
FA
FC
|
| 13-1351 |
Biotin anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72) |
N/A |
N/A |
FC
|
| 12-1351 |
PE anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72) |
488 |
575 |
FC
|
| 15-1351 |
Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72) |
488 |
670 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3. A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow. Two-color staining of adult mouse bone marrow cells with A2F10 and
RA3-6B2 (CD45R/B220) or
M1/70 (CD11b) reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The A2F10 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ogawa, M., S. Sugawara, et al. (1998). "Flt3/Flk-2 and c-Kit are not essential for the proliferation of B lymphoid progenitor cells in the bone marrow of the adult mouse." Exp Hematol 26(6): 478-88.
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Mouse Cellular Antigens Chart