Product Information

Contents: Biotin anti-human CD123 (interleukin-3 receptor α, IL-3Rα)
Catalog Number: 13-1239
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 6H6
Isotype: Mouse IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1239	Affinity Purified anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	N/A	N/A	FC IH/F IHC(Paraffin)
13-1239	Biotin anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	N/A	N/A	FC
11-1239	Fluorescein isothiocyanate (FITC) anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra)	488	518	FC
12-1239	PE anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	488	575	FC
17-1239	Allophycocyanin (APC) anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	633	660	FC
25-1239	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	488	760	FC
45-1239	PerCP-Cy5.5 anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra)	488	690	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 6H6 monoclonal antibody reacts with human CD123, the α chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common β chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 6H6 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 6H6 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Herling, M., M. Teitell, R. Shen, L. Medeiros, and D. Jones. 2003. TCL1 expression in plasmacytoid dendritic cells (DC2s) and the related CD4+ CD56+ blastic tumors of skin. Blood. 101: 5007-5009.

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