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Mouse Cellular Antigens Chart

Contents: Biotin anti-mouse CD123 (interleukin-3 receptor α, IL-3Rα) Catalog Number: 13-1231 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. DO NOT FREEZE. Clone: 5B11 Isotype: Rat IgG2a, κ Prices for This Product* Cat. No. Size Price Qty Action 13-1231-81 50 ug $85 13-1231-82 100 ug $155 13-1231-85 500 ug $260 *International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-1231	Fluorescein isothiocyanate (FITC) anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	488	518	FC
12-1231	PE anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	488	575	FC
17-1231	Allophycocyanin (APC) anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra)	633	660	FC
14-1231	Affinity Purified anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	N/A	N/A	FC
13-1231	Biotin anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The 5B11 monoclonal antibody reacts with mouse CD123, the α chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself and when associated with either CD131 (common β chain) or AIC2A (IL-3β) binds IL-3 with high affinity. CD123 does not transduce any intracellular signals upon binding IL-3 and requires the β chain for this function.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The 5B11 antibody has been reported for use in flow cytometric analysis.

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Applications Tested

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The 5B11 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Ichihara, M., T. Hara, et al. (1995). "Impaired interleukin-3 (IL-3) response of the A/J mouse is caused by a branch point deletion in the IL-3 receptor alpha subunit gene." Embo J 14(5): 939-50.
Mueller, D. L., Z. M. Chen, et al. (1994). "Subset of CD4+ T cell clones expressing IL-3 receptor alpha-chains uses IL-3 as a cofactor in autocrine growth." J Immunol 153(7): 3014-27.

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Related Products

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Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4321    Biotin Rat IgG2a Isotype Control