Product Information

Contents: Biotin anti-human CD115 (CSF-1R, FMS, CSF-1 Receptor)
Catalog Number: 13-1159
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 12-3A3-1B10
Isotype: Rat IgG1

Staining of normal human peripheral blood cells with 0.5 μg of Biotin Rat IgG1 Iso Cntrl (cat. 13-4301) (open histogram) or 0.5 μg of Biotin anti-human CD115 (12-3A3-1B10) (colored histogram) followed by SAv-PE (cat. 12-4317). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1159	Affinity Purified anti-human CD115 (CSF-1R, FMS, CSF-1 Receptor)	N/A	N/A	FC IHC IP WB
13-1159	Biotin anti-human CD115 (CSF-1R, FMS, CSF-1 Receptor)	N/A	N/A	FC
12-1159	Phycoerythrin (PE) anti-human CD115 (CSF-1R, FMS, CSF-1 Receptor)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

CD115 or Colony-Stimulating Factor-1 Receptor (CSF-1R) is expressed on cells committed to the monocyte lineage and osteoclasts. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subsequently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. CSF-1R activation promotes the survival, proliferation and differentiation of mononuclear phagocytes and the spreading and motility of macrophages. In osteoclasts, CSF-1R synergizes with RANKL to regulate the differentiation of mononuclear phagocytes to osteoclasts.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 12-3A3-1B10 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 12-3A3-1B10 antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.06 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ashmun RA, Look AT, Roberts WM, Roussel MF, Seremetis S, Ohtsuka M, Sherr CJ. Monoclonal antibodies to the human CSF-1 receptor (c-fms proto-oncogene product) detect epitopes on normal mononuclear phagocytes and on human myeloid leukemic blast cells. Blood. 1989 Feb 15;73(3):827-37. (12-3A3-1B10, FC, PubMed)

Pixley FJ, Stanley ER. CSF-1 regulation of the wandering macrophage: complexity in action. Trends Cell Biol. 2004 Nov;14(11):628-38. Review.

Related Products

Cat. 13-4301  Biotin Rat IgG1 Isotype Control
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)