Staining of normal human peripheral blood cells with Anti-Human CD4 FITC (cat. 11-0048) and 0.25 µg of Mouse IgG1 κ Isotype Control Biotin (cat. 13-4714) (left) or 0.25 µg of Anti-Human CD101 Biotin (right) followed by Streptavidin PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Human CD101 Biotin Catalog Number: 13-1019 Concentration: 0.5 mg/ml Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Clone: BB27 Host/Isotype: Mouse IgG1
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Size
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13-1019-80
25 ug
13-1019-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The monoclonal antibody BB27 reacts to human CD101 also known as V7 antigen, a member of the Ig superfamily. CD101 is a disulfide linked homodimer of unknown function. Expression of CD101 is found on monocytes, granulocytes and dendritic cells (Langerhan-like cells HLA-DR, CD1a, CD1c). In addition expression on T lymphocytes is important for cell activation. CD101+ CD28+ cells are very responsive to CD28 signaling. In combination with anti-CD28 or suboptimal levels of anti-CD3, anti-CD101 can increase proliferation thereby suggesting an activating role. The monoclonal antibody BB27 has been shown to inhibit the T cell reactivity in allogeneic and antigen-specific mixed DC-T cell cultures.
Recently it has been demonstrated that mouse CD101 is found on a subpopulation of regulatory T cells (CD4, CD25, Foxp3 positive) that have high suppressor activity. Expression of CD101 on human PBMCs shows staining of about 30% of the Foxp3 positive cells. Studies have not confirmed higher suppressor activity in the human CD101 population.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This BB27 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This BB27 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Gouttefangeas C, Jacquot S, Meffre E, Schmid M, Boumsell L, Bensussan A. Differential proliferative responses in subsets of human CD28+ cells delineated by BB27 mAb.
Int Immunol. 1994 Mar;6(3):423-30. (BB27, FC, PubMed)
Bouloc A, Bagot M, Delaire S, Bensussan A, Boumsell L. Triggering CD101 molecule on human cutaneous dendritic cells inhibits T cell proliferation via IL-10 production.
Eur J Immunol. 2000 Nov;30(11):3132-9. (BB27, FA, PubMed)
Bouloc A, Boulland ML, Geissmann F, Fraitag S, Andry P, Teillac D, Bensussan A, Revuz J, Boumsell L, Wechsler J, Bagot M. CD101 expression by Langerhans cell histiocytosis cells.
Histopathology. 2000 Mar;36(3):229-32. {BB27, Ihf, PubMed)
