Staining of BALB/c splenocytes with 0.06 µg of Rat IgG2a κ Isotype Control Biotin (cat. 13-4321) (open histogram) or 0.06 µg of Anti-Mouse CD94 Biotin (filled histogram) followed by Streptavidin PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Mouse CD94 Biotin Catalog Number: 13-0941 Concentration: 0.5 mg/ml Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Clone: 18d3 Host/Isotype: Rat IgG2a, κ
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Size
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13-0941-81
50 ug
13-0941-82
100 ug
13-0941-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 18d3 monoclonal antibody reacts with mouse CD94, a 70 kDa type II transmembrane glycoprotein. CD94 belongs to the C-type lectin superfamily and is present as a heterodimer with NKG2 on the surface. CD94 is expressed by NK cells, a subset of T cells, and NKT cells and plays an important role in adhesion and activation of NK cell lineage. DBA/2J mice are naturally CD94-deficient and do not express cell-surface CD94/NKG2A receptors, even on neonatal NK cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 18d3 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 18d3 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Vance, R. E., A. M. Jamieson, et al. (1999). "Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells." J Exp Med 190(12): 1801-12. Vance, R. E., J. R. Kraft, et al. (1998). "Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b)." J Exp Med 188(10): 1841-8. Vance, R. E., D. M. Tanamachi, et al. (1997). "Cloning of a mouse homolog of CD94 extends the family of C-type lectins on murine natural killer cells." Eur J Immunol 27(12): 3236-41.
