Product Information

Contents: Biotin anti-human CD93 (C1qRp)
Catalog Number: 13-0938
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: R3
Isotype: Mouse IgM

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or 0.5 μg of Biotin anti-human CD93 (R3) (colored histogram) followed by SAv-PE (cat. 12-4317). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-0938	Biotin anti-human CD93 (C1qRp)	N/A	N/A	ELISA det FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody R3 recognizes human CD93, also known as C1qRp. The glycoprotein CD93 binds to C1q, the subunit of the complement protein, mannose binding lectin and pulmonary surfactant protein A. CD93 is predicted to play a role in the clearance of apoptotic cells. Expression of CD93 is confined to myeloid cells with higher expression on monocytes than neutrophils, eosinophils, platelets and endothelial cells. Expression on DC’s is downregulated upon maturation. Additionally, CD93 has been shown to define an early bone marrow stem cell population of hematopoietic and hepatic precursors.
The R3 antibody has been shown to have activating function by increasing phagocytosis when presented in an immobilized state and inhibitory functions when presented in a soluble state. CD93 can be shed from the cell surface. This phenomenon can be measures using R3 antibody as detection with R139 as capture to detect soluble CD93 by ELISA. The epitope for R3 lies in the CRD domain.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This R3 antibody has been reported for use in flow cytometric analysis, and ELISA (detection).

Applications Tested

This R3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Bohlson SS, Silva R, Fonseca MI, Tenner AJ. CD93 is rapidly shed from the surface of human myeloid cells and the soluble form is detected in human plasma. J Immunol. 2005 Jul 15;175(2):1239-47. (R3, ELISA, WB, PubMed)

Danet GH, Luongo JL, Butler G, Lu MM, Tenner AJ, Simon MC, Bonnet DA. C1qRp defines a new human stem cell population with hematopoietic and hepatic potential. Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10441-5.(R3, FC, PubMed)

Nepomuceno RR, Tenner AJ. C1qRP, the C1q receptor that enhances phagocytosis, is detected specifically in human cells of myeloid lineage, endothelial cells, and platelets. J Immunol. 1998 Feb 15;160(4):1929-35. (R3, FC, PubMed)

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Cat. 13-5892  Biotin anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen) (clone AA4.1)