Product Information

Contents: Biotin anti-human CD80 (B7-1)
Catalog Number: 13-0809
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 2D10.4
Isotype: Mouse IgG1, κ
HLDA No.: VI B-CD80.1

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0809	Affinity Purified anti-human CD80 (B7-1, B71)	N/A	N/A	FC
16-0809	Functional Grade* Purified anti-human CD80 (B7-1, B71)	N/A	N/A	FA FC
13-0809	Biotin anti-human CD80 (B7-1, B71)	N/A	N/A	FC
11-0809	FITC anti-human CD80 (B7-1, B71)	488	518	FC
12-0809	PE anti-human CD80 (B7-1, B71)	488	575	FC
15-0809	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD80 (B7-1, B71)	488	670	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 2D10.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 2D10.4 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Bashuda, H., Kimikawa, M., Seino, K., Kato, Y., Ono, F., Shimizu, A., Yagita, H., Teraoka, S., and K. Okumura. 2005. Renal allograft rejection is prevented by adoptive transfer of anergic T cells in nonhuman primates. J Clin Invest.115:1896-1902. (2D10.4, FA, PubMed)

Kishimoto, T., A.E.G., von dem Borne, et al. eds. 1998 .Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.

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