Also known as: Invariant chain, MHC Class II-associated, Ia-gamma, Receptor for Macrophage Migration Inhibitory Factor
RUO: For Research Use Only
Contents: Anti-Human CD74 Biotin Catalog Number: 13-0749 Concentration: 0.1 mg/ml Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization Storage Conditions: Store at 2-8°C. Do not freeze. Clone: LN2 Host/Isotype: Mouse IgG1, κ
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Size
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13-0749-71
25 tests
13-0749-73
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The LN2 monoclonal antibody recognizes CD74 (MHC Class II-associated invariant chain), which is a 33-43 kDa nonpolymorphic type II membrane protein. CD74 is expressed on B cells, at lower levels on monocytes and macrophages, and on subsets of activated T cells, Langerhans cells, dendritic cells and epithelial cells. CD74 is known to act as a chaperone of MHC Class II (HLA-DR) proteins, promoting their exit from the ER and directing them to endocytic compartments. However, recently a signaling function has been demonstrated for CD74 based on its interaction with CD44 and its binding of the proinflammatory cytokine macrophage migration-inhibitory factor (MIF). CD74 is also thought to play a role in the maturation of follicular B-cells and accumulation of marginal zone B-cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This LN2 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections.
Applications Tested
This LN2 antibody has been pre-titrated and tested by flow cytometric analyis of normal human peripheral blood leukocytes. This can be used at 10 μl per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Epstein AL, Marder RJ, Winter JN, Fox RI.
Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors.
J Immunol. 1984 Aug;133(2):1028-36. (LN2, IHC (Paraffin), IP, PubMed)
Lamb CA, Yewdell JW, Bennink JR, Cresswell P.
Invariant chain targets HLA class II molecules to acidic endosomes containing internalized influenza virus.
Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):5998-6002.
Becker-Herman S, Arie G, Medvedovsky H, Kerem A, Shachar I.
CD74 is a member of the regulated intramembrane proteolysis-processed protein family.
Mol Biol Cell. 2005 Nov;16(11):5061-9.
