Product Information

Contents: Biotin anti-human CD71 (transferrin receptor)
Catalog Number: 13-0719
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: OKT9 (OKT-9)
Isotype: Mouse IgG1

Staining of 3-day unstimulated (left) and 3-day PHA activated (right) human peripheral blood leukocytes with 0.25 μg of Biotin Mouse IgG1, K Iso Cntrl (cat. 13-4714) (open histogram) or 0.06 μg of Biotin OKT9 (OKT-9) (colored histogram) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0719	Affinity Purified anti-human CD71 (transferrin receptor)	N/A	N/A	FC
16-0719	Functional Grade* Purified anti-human CD71 (transferrin receptor)	N/A	N/A	FC
13-0719	Biotin anti-human CD71 (transferrin receptor)	N/A	N/A	FC
11-0719	Fluorescein isothiocyanate (FITC) anti-human CD71 (transferrin receptor)	488	518	FC
12-0719	Phycoerythrin (PE) anti-human CD71 (transferrin receptor)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The OKT9 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71.

Applications Reported

For research use only, not for diagnostic or therapeutic use.

Applications Tested

This OKT9 (OKT-9) antibody has been tested by flow cytometric analysis of unstimulated and PHA-activated (3 days) human blood leukocytes. This can be used at less than or equal to 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sutherland R, Delia D, Schneider C, Newman R, Kemshead J, Greaves M. 1981. Ubiquitous cell-surface glycoprotein on tumor cells is proliferation-associated receptor for transferrin. Proc Natl Acad Sci U S A. 78(7): 4515–4519.

Salcedo TW, Fleit HB. 1991. Plasma membrane and intracellular pools of transferrin receptors decline during in vitro cultivation of U937 cells. Cell Prolif. 24(4):383-401.

Wenning LA, Murphy RM. 1999. Coupled cellular trafficking and diffusional limitations in delivery of immunotoxins to multicell tumor spheroids. Biotechnol Bioeng. 62(5):562-75.

Related Products

Cat. 11-0717  Fluorescein isothiocyanate (FITC) anti-human CD71 (Transferrin Receptor) (clone T56/14)
Cat. 12-0717  Phycoerythrin (PE) anti-human CD71 (Transferrin Receptor) (clone T56/14)
Cat. 14-0717  Affinity Purified anti-human CD71 (Transferrin Receptor) (clone T56/14)