Product Information

Contents: Biotin anti-mouse CD66a (CEACAM, CEACAM1a)
Catalog Number: 13-0661
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: CC1
Isotype: Mouse IgG1

Staining of C57Bl/6 splenocytes with 0.03 μg of Biotin anti-mouse CD66a (CC1) followed by SAv-PE (cat. 12-4317) and Pacific Blue anti-mouse CD3e (17A2) (cat. 57-0032)(left) or FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) (right). Quadrant lines denote isotype control levels. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0661	Affinity Purified anti-mouse CD66a (CEACAM , CEACAM1a)	N/A	N/A	FA FC IHC(Paraffin)
13-0661	Biotin anti-mouse CD66a (CEACAM, CEACAM1a)	N/A	N/A	FC IHC(Paraffin)
12-0661	Phycoerythrin (PE) anti-mouse CD66a (CEACAM, CEACAM1a)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody CC1 recognizes CD66a, also known as carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), biliary glycoprotein, and BPG. Expression of CD66a is found on brush borders, epithelial, and endothelial cells. In hematopoietic cells expression is found abundantly on B cells, as well as some NKs, monocytes, DCs, and granulocytes. Although low levels of mRNA have been identified in T cells in humans, resting mouse T lymphocytes are not reported to express CD66a, as confirmed by lack of staining with CC1 antibody. In humans, expression levels of CD66a have been used to identify malignancies. CD66a plays a key role as a regulator of BCR activation of B lymphocytes.

An alternate allele, CEACAM1b, is expressed in SJL mice; therefore, CC1 does not stain SJL tissue. The monoclonal CC1 has been shown to block viral infection and also enhance B cell proliferation when combined with IgM crosslinking.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This CC1 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of paraffin embedded tissue sections.

Applications Tested

This CC1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Thirion G, Feliu AA, Coutelier JP. CD66a (CEACAM1) expression by mouse natural killer cells. Immunology. 2008 May 17. (CC1, FC, PubMed)

Nakagaki K, Nakagaki K, Taguchi F. Receptor-independent spread of a highly neurotropic murine coronavirus JHMV strain from initially infected microglial cells in mixed neural cultures. J Virol. 2005 May;79(10):6102-10. (CC1, FA, FC PubMed)

Turner BC, Hemmila EM, Beauchemin N, Holmes KV. Receptor-dependent coronavirus infection of dendritic cells. J Virol. 2004 May;78(10):5486-90. (CC1, FA, FC, PubMed)

Greicius G, Severinson E, Beauchemin N, Obrink B, Singer BB. CEACAM1 is a potent regulator of B cell receptor complex-induced activation. J Leukoc Biol. 2003 Jul;74(1):126-34. (CC1, FC, PubMed)

Kammerer R, Stober D, Singer BB, Obrink B, Reimann J. Carcinoembryonic antigen-related cell adhesion molecule 1 on murine dendritic cells is a potent regulator of T cell stimulation. J Immunol. 2001 Jun 1;166(11):6537-44

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