Contents: Biotin anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)
Catalog Number: 13-0621
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: MEL-14
Isotype: Rat IgG2a, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of C57Bl/6 splenocytes with 0.125 μg Biotin Rat IgG2a Iso Cntrl (cat. 13-4321) (open histogram) or 0.125 μg Biotin anti-mouse CD62L (MEL-14) (colored histogram) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-0621 |
Affinity Purified anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) |
N/A |
N/A |
FA
FC
IHC
IP
|
| 16-0621 |
Functional Grade* Purified anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) |
N/A |
N/A |
FA
FC
|
| 13-0621 |
Biotin anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) |
N/A |
N/A |
FC
|
| 11-0621 |
FITC anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) |
488 |
518 |
FC
|
| 12-0621 |
PE anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) |
488 |
575 |
FC
|
| 15-0621 |
PE-Cy5 anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) |
488 |
670 |
FC
|
| 17-0621 |
APC anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) |
633 |
660 |
FC
|
| 25-0621 |
Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) |
488 |
760 |
FC
|
| 35-0621 |
Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) |
488 |
690 |
FC
|
| 56-0621 |
Alexa Fluor® 700 anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
| 57-0621 |
Pacific Blue® anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) |
402 |
421 |
FC
|
| 27-0621 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
| 45-0621 |
PerCP-Cy5.5 anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) |
488 |
690 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The MEL-14 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Gallatin, W. M., I. L. Weissman, et al. (1983). "A cell-surface molecule involved in organ-specific homing of lymphocytes."
Nature 304(5921): 30-4.
Siegelman, M. H., I. C. Cheng, et al. (1990). "The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding."
Cell 61(4): 611-22.
Related Products
Cat.
11-4317 Streptavidin-FITC (Fluorescein isothiocyanate)
Cat.
12-4317 Streptavidin-PE (Phycoerythrin)
Cat.
17-4317 Streptavidin Allophycocyanin (SA-APC)
Cat.
13-4321 Biotin Rat IgG2a Isotype Control
Mouse Cellular Antigens Chart