Product Information

Contents: Biotin anti-rat Granulocytes
Catalog Number: 13-0570
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: HIS48
Isotype: Mouse IgM

Surface staining of rat splenocytes with anti-rat Granulocytes (HIS48) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0570	Affinity Purified anti-rat Granulocytes	N/A	N/A	FC IH/F
13-0570	Biotin anti-rat Granulocytes	N/A	N/A	FC
11-0570	FITC anti-rat Granulocytes	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HIS48 monoclonal antibody reacts with a molecule expressed on the surface of all rat granulocytes. The antigen recognized by HIS48 is also expressed by erythroid lineage cells during their developmental stages in the bone marrow. HIS48 is commonly used as a phenotypic marker for rat granulocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIS48 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HIS48 antibody has been tested by flow cytometric analysis of rat splenocyte suspensions. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Rhodes, N. P., J. A. Hunt, et al. 1997. Macrophage subpopulation differentiation by stimulation with biomaterials. J Biomed Mater Res 37(4): 481-8.
Badger, D. A., J. M. Sauer, et al. 1996. The role of inflammatory cells and cytochrome P450 in the potentiation of CCl4-induced liver injury by a single dose of retinol. Toxicol Appl Pharmacol 141(2): 507-19.
Clark, W. M., J. D. Lauten, et al. 1995. Time course of ICAM-1 expression and leukocyte subset infiltration in rat forebrain ischemia. Mol Chem Neuropathol 26(3): 213-30.
Van Goor, H., et al. 1991. Determinants of focal and segmental glomerulosclerosis in the rat after renal ablation. Evidence for involvement of macrophages and lipids. Lab. Invest. 64: 754-765.

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