Product Information

Contents: Biotin anti-human CD54 (ICAM-1)
Catalog Number: 13-0549
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: HA58
Isotype: Mouse IgG1, κ
HLDA No.: VI A095

Normal human peripheral blood stained with biotinylated HA58 and detected with Streptavidin-PE (cat.no. 12-4317). Cells in the lymphocyte gate were analyzed.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0549	Affinity Purified anti-human CD54 (ICAM-1)	N/A	N/A	FA FC IHC WB
16-0549	Functional Grade* Purified anti-human CD54 (ICAM-1)	N/A	N/A	FA FC
13-0549	Biotin anti-human CD54 (ICAM-1)	N/A	N/A	FC
12-0549	PE anti-human CD54 (ICAM-1)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligand CD11a is important in the inflammatory response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HA58 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HA58 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Schlossman, S., L. Bloumsell, et al. eds 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Kishimoto, T., H. Kikutani, et al., eds. 1998. Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.

Kanwar JR, Berg RW, et al. 2003. Requirements for ICAM-1 immunogene therapy of lymphoma. Cancer Gene Ther. 10(6):468-76. (WB, PubMed)

Bao J, Sato K, et al. 2001. PR-39 and PR-11 peptides inhibit ischemia-reperfusion injury by blocking proteasome-mediated I kappa B alpha degradation. Am J Physiol Heart Circ Physiol. 281(6):H2612-8. (WB, PubMed)

Davis DM, Chiu I, et al. 1999. The human natural killer cell immune synapse. Proc Natl Acad Sci U S A. 96(26):15062-7. (IHC, PubMed)

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