Product Information

Contents: Biotin anti-mouse CD54 (ICAM-1)
Catalog Number: 13-0541
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: YN1/1.7.4
Isotype: Rat IgG2b, κ

Staining of C57Bl/6 splenocytes with 0.06 μg of Biotin Rat IgG2b Iso Cntrl (cat. 13-4331) (open histogram) or 0.06 μg of Biotin anti-mouse CD54 (YN1/1.7.4) (colored histogram) followed by SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0541	Affinity Purified anti-mouse CD54 (ICAM-1)	N/A	N/A	FA FC IHC
16-0541	Functional Grade* Purified anti-mouse CD54 (ICAM-1)	N/A	N/A	FA FC
13-0541	Biotin anti-mouse CD54 (ICAM-1)	N/A	N/A	FC
11-0541	FITC anti-mouse CD54 (ICAM-1)	488	518	FC
12-0541	PE anti-mouse CD54 (ICAM-1)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response. The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monclonal antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The YN1/1.7.4 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Burns, A. R., F. Takei, et al. (1994). "Quantitation of ICAM-1 expression in mouse lung during pneumonia." J Immunol 153(7): 3189-98.
Fabry, Z., M. M. Waldschmidt, et al. (1992). "Adhesion molecules on murine brain microvascular endothelial cells: expression and regulation of ICAM-1 and Lgp 55." J Neuroimmunol 36(1): 1-11.
Jevnikar, A. M., R. P. Wuthrich, et al. (1990). "Differing regulation and function of ICAM-1 and class II antigens on renal tubular cells." Kidney Int 38(3): 417-25.
Kumasaka, T., W. M. Quinlan, et al. (1996). "Role of the intercellular adhesion molecule-1(ICAM-1) in endotoxin-induced pneumonia evaluated using ICAM-1 antisense oligonucleotides, anti-ICAM-1 monoclonal antibodies, and ICAM-1 mutant mice." J Clin Invest 97(10): 2362-9.

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