Product Information

Contents: Biotin anti-human CD51/61
Catalog Number: 13-0519
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 23C6
Isotype: Mouse IgG1, κ
HLDA No.: IV P18

Staining of M21 cells with 0.5 μg of Biotin Mouse IgG1, K Iso Cntrl (cat. 13-4714) (open histogram) or 0.5 μg of Biotin anti-human CD51/CD61 (23C6) (colored histogram) followed by Streptavidin-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0519	Affinity Purified anti-human CD51/61	N/A	N/A	FC IH/F
16-0519	Functional Grade* Purified anti-human CD51/61	N/A	N/A	FA FC IH/F
13-0519	Biotin anti-human CD51/61	N/A	N/A	FC
11-0519	FITC anti-human CD51/61	488	518	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin αv/β3. CD51, an ~120 kDa surface molecule can also non-covalently associate with other β subunits of the integrin family including β₁ (CD29), β₅ and β₆ to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by melanoma cells, endothelial cells and osteoclasts and at very low levels by platelets. The CD51/CD61 complex mediates adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 23C6 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 23C6 antibody has been tested by flow cytometric analysis of peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4714    Biotin Mouse IgG1, K Isotype Control