Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (cat. 11-0038) and 0.25 µg of Mouse IgG1 κ Isotype Control Biotin (cat. 13-4714) (left) or 0.25 µg of Anti-Human CD49e (Integrin α5) Biotin (right) followed by Streptavidin PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Human CD49e (Integrin alpha 5) Biotin Catalog Number: 13-0496 Concentration: 0.5 mg/ml Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Clone: eBioSAM-1 (SAM-1, SAM1) Host/Isotype: Mouse IgG1
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Size
Price
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13-0496-80
25 ug
13-0496-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The eBioSAM-1 monoclonal antibody reacts with human integrin alpha 5, also known as fibronectin receptor alpha chain, very late activation antigen 5 alpha, and CD49e. Integrins are composed of an alpha chain and a beta chain, which non-covalently associate to form the functional integrin. Integrin heterodimers participate in cell surface-mediated signaling and adhesion functions. Integrin alpha 5 undergoes post-translational cleavage in its extracellular domain to yield disulfide linked light and heavy chains that join with Integrin beta 1 (CD29) to form the fibronectin receptor, also known as the very late activation antigen-5 (VLA-5) complex. Integrin alpha 5 is expressed on thymocytes, T cells, monocytes, platelets, early B cells, and activated B cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioSAM-1 (SAM-1, SAM1) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioSAM-1 (SAM-1, SAM1) antibody has been tested by flow cytometric analysis of human PBMCs. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Xiao J, Messinger Y, Jin J, Myers DE, Bolen JB, Uckun FM. Signal transduction through the beta1 integrin family surface adhesion molecules VLA-4 and VLA-5 of human B-cell precursors activates CD19 receptor-associated protein-tyrosine kinases. J Biol Chem. 1996 Mar 29;271(13):7659-64. (SAM-1, FC, PubMed)
Grassi F, Dezutter-Dambuyant C, McIlroy D, Jacquet C, Yoneda K, Imamura S, Boumsell L, Schmitt D, Autran B, Debre P, Hosmalin A. Monocyte-derived dendritic cells have a phenotype comparable to that of dermal dendritic cells and display ultrastructural granules distinct from Birbeck granules. J Leukoc Biol. 1998 Oct;64(4):484-93. (SAM-1, FC, IHC, PubMed)
Santoni G, Birarelli P, Hong LJ, Gamero A, Djeu JY, Piccoli M. An alpha 5 beta 1-like integrin receptor mediates the binding of less pathogenic Candida species to fibronectin. J Med Microbiol. 1995 Nov;43(5):360-7. (SAM-1, FC, PubMed)
Theodore PR, Simon AR, Warrens AN, Sackstein R, Sykes M. Porcine mononuclear cells adhere to human fibronectin independently of very late antigen-5: implications for donor-specific tolerance induction in xenotransplantation. Xenotransplantation. 2002 Jul;9(4):277-89. (SAM-1, FA, PubMed)
