Product Information

Contents: Biotin anti-mouse CD49b (Integrin α₂)
Catalog Number: 13-0491
Sizes: 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: HMa2
Isotype: Armenian Hamster IgG

Staining of BALB/c splenocytes with 0.125 μg of Biotin Armenian Hamster IgG Iso Cntrl (cat. 13-4888) (open histogram) or 0.125 μg of Biotin anti-mouse CD49b (HMa2) (colored histogram) followed by SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0491	Affinity Purified anti-mouse CD49b (Integrin alpha 2)	N/A	N/A	FA FC IHC IP
16-0491	Functional Grade* Purified anti-mouse CD49b (Integrin alpha 2)	N/A	N/A	FA FC
13-0491	Biotin anti-mouse CD49b (Integrin alpha 2)	N/A	N/A	FC
11-0491	FITC anti-mouse CD49b (Integrin alpha 2)	488	518	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HMα2 monoclonal antibody reacts with mouse CD49b, the 150 kDa integrin α₂ subunit. The complex of CD49b non-covalently associated with integrin β₁ (CD29), also known as VLA-2, is a receptor for collagen and laminin. This complex is expressed by some CD4⁺ T cells, IEL, NK cells, platelets and epithelial cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HMα2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HMα2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Miyake, S., T. Sakurai, et al. (1994). "Identification of collagen and laminin receptor integrins on murine T lymphocytes." Eur J Immunol 24(9): 2000-5.
Noto, K., K. Kato, et al. (1995). "Identification and functional characterization of mouse CD29 with a mAb." Int Immunol 7(5): 835-42.

Related Products

Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4888    Biotin Armenian Hamster IgG Isotype Control (clone eBio299Arm)