Product Information

Contents: Biotin anti-mouse CD48 (BCM1)
Catalog Number: 13-0481
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: HM48-1
Isotype: Armenian Hamster IgG

Staining of C57Bl/6 splenocytes with .06 μg of Biotin Armenian Hamster IgG Iso Cntrl (cat. 13-4888) (open histogram) or .5 μg of Biotin anti-mouse CD48 (HM48-1) (colored histogram) followed by Streptavidin-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0481	Affinity Purified anti-mouse CD48 (BCM1)	N/A	N/A	FC IP WB
16-0481	Functional Grade* Purified anti-mouse CD48 (BCM1)	N/A	N/A	FA IP WB
13-0481	Biotin anti-mouse CD48 (BCM1)	N/A	N/A	FC
11-0481	Fluorescein isothiocyanate (FITC) anti-mouse CD48 (BCM1)	488	518	FC
12-0481	Phycoerythrin (PE) anti-mouse CD48 (BCM1)	488	575	FC
17-0481	Allophycocyanin (APC) anti-mouse CD48 (BCM1)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HM48-1 monoclonal antibody reacts with the mouse CD48 antigen; also known as BCM1, Blast-1 (human), and OX-45 (rat). CD48, a member of the SLAM family and Ig superfamily, is a 45 kDa GPI-linked glycoprotein expressed on the majority of hematopoietic cells. Recent publications have reported differential expression of members of the SLAM family including CD48, CD150, and CD244 among functionally distinct bone marrow hematopoietic progenitors providing a useful tool for prediction of the primitiveness of hematopoietic progenitors based on the expression of these SLAM family members. Hematopoietic stem cells (HSC) are highly purified as CD150(+)CD244(-)CD48(-) cells while non-self-renewing multipotent hematopoietic progenitors (MPP) are CD244(+)CD150(-)CD48(-) and the most restricted progenitors are CD48(+)CD244(+)CD150(-). CD48 plays a critical role in adhesion and T cell activation. In the mouse, the primary counter-receptors for CD48 are CD2 and CD244. HM48-1 is reported to modulate in vitro and in vivo CD48 functions including blocking the CD48/CD2 and CD48/CD244 interactions, inhibiting the proliferative response of mitogen-activated spleen cells, providing a costimulation signal for T cells activated in vitro through their TCR, and prolonging cardiac allograft survival in vivo.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HM48-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HM48-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Chavin KD, Qin L, Lin J, Woodward J, Baliga P, Kato K, Yagita H, Bromberg JS. Anti-CD48 (murine CD2 ligand) mAbs suppress cell mediated immunity in vivo. Int Immunol. 1994 May;6(5):701-9.

Kato K, Tamura N, Okumura K, Yagita H. Identification of the T cell surface signal-transducing glycoprotein sgp-60 as CD48, a counter-receptor for mouse CD2. Eur J Immunol. 1993 Jun;23(6):1412-5.

Kato K, Koyanagi M, Okada H, Takanashi T, Wong YW, Williams AF, Okumura K, Yagita H. CD48 is a counter-receptor for mouse CD2 and is involved in T cell activation. J Exp Med. 1992 Nov 1;176(5):1241-9.

Kiel MJ, Yilmaz OH, Iwashita T, Yilmaz OH, Terhorst C, Morrison SJ. SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Cell. 2005. 121:1109-21.

Related Products

Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4888    Biotin Armenian Hamster IgG Isotype Control (clone eBio299Arm)