Product Information

Contents: Biotin anti-rat CD45R
Catalog Number: 13-0460
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: HIS24
Isotype: Mouse IgG2b, κ

Surface staining of rat splenocytes with anti-rat CD45R (HIS24) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0460	Affinity Purified anti-rat CD45R	N/A	N/A	FC IHC WB
13-0460	Biotin anti-rat CD45R	N/A	N/A	FC
11-0460	FITC anti-rat CD45R	488	518	FC
12-0460	PE anti-rat CD45R	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HIS24 monoclonal antibody reacts with an isoform of CD45 expressed by rat B cells. The antigen recognized by HIS24 is found on B cells from the early stage of B cell development in the bone marrow to mature peripheral B cells, but not on plasma cells. HIS24 recognizes a 205 kDa protein by immunoblotting. CD45, also known as Leukocyte Common Antigen (LCA), has tyrosine phosphatase enzymatic activity in its cytoplasmic portion and plays an important role in activation of lymphocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIS24 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HIS24 antibody has been tested by flow cytometric analysis of rat splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kroese, F. G., D. Opstelten, et al. 1985. Monoclonal antibodies to rat B lymphocyte (sub-) populations. Adv Exp Med Biol 186: 81-9.
Kroese, F. G., A. S. Wubbena, et al. 1987. B lymphocyte differentiation in the rat: production and characterization of monoclonal antibodies to B lineage-associated antigens. Eur J Immunol 17(7): 921-8.
Kroese, F. G., E. C. Butcher, et al. 1990. The rat B cell system: the anatomical localization of flow cytometry-defined B cell subpopulations. Eur J Immunol 20(7): 1527-34.

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