Contents: Biotin anti-human CD45 (LCA)
Catalog Number: 13-0459
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: HI30
Isotype: Mouse IgG1, κ
HLDA No.: IV N816
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Surface staining of normal human peripheral blood cells with anti-human CD45 (HI30) PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
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Description
The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The HI30 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The HI30 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
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Human Cellular Antigens Chart