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Mouse Cellular Antigens Chart

Contents: Biotin anti-mouse CD45RB Catalog Number: 13-0455 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. DO NOT FREEZE. Clone: C363.16A Isotype: Rat IgG2a, κ Prices for This Product* Cat. No. Size Price Qty Action 13-0455-81 50 ug $55 13-0455-82 100 ug $80 13-0455-85 500 ug $260 *International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c splenocytes with 0.06 μg of Biotin Rat IgG2a Iso Cntrl (cat. 13-4321) (open histogram) or 0.06 μg of Biotin anti-mouse CD45RB (C363.16A) (colored histogram) followed by SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0455	FITC anti-mouse CD45RB	488	518	FC
12-0455	PE anti-mouse CD45RB	488	575	FC
13-0455	Biotin anti-mouse CD45RB	N/A	N/A	FC
14-0455	Affinity Purified anti-mouse CD45RB	N/A	N/A	FC  IH/F  IP
17-0455	Allophycocyanin (APC) anti-mouse CD45RB	633	660	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The C363.16A monoclonal antibody reacts with the exon B-dependent isoform of mouse CD45. This CD45 isoform is expressed by thymocytes, peripheral B cells and a subpopulation of T cells at high density and by dendritic cells and macrophages at low density. CD45 is a protein tyrosine phosphatase and serves an important role in leukocyte signal transduction.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The C363.16A antibody has been reported for use in flow cytometric analysis.

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Applications Tested

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The C363.16A antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Bottomly, K., M. Luqman, et al. (1989). "A monoclonal antibody to murine CD45R distinguishes CD4 T cell populations that produce different cytokines." Eur J Immunol 19(4): 617-23.
Johnson, P., L. Greenbaum, et al. (1989). "Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies." J Exp Med 169(3): 1179-84.

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Related Products

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Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4321    Biotin Rat IgG2a Isotype Control