Contents: Biotin anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)
Catalog Number: 13-0454
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 104
Isotype: Mouse IgG2a, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Surface staining of BALB/c mouse splenocytes with anti-mouse CD45.2 (104) PE (left), and APC-Cy7 (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-0454 |
FITC anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
488 |
518 |
FC
|
| 12-0454 |
PE anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
488 |
575 |
FC
|
| 13-0454 |
Biotin anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
N/A |
N/A |
FC
IH/F
|
| 14-0454 |
Affinity Purified anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
N/A |
N/A |
FC
IH/F
IP
|
| 17-0454 |
Allophycocyanin (APC) anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
633 |
660 |
FC
|
| 27-0454 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
| 35-0454 |
PE-Cy5.5 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
488 |
690 |
FC
|
| 45-0454 |
PerCP-Cy5.5 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
488 |
690 |
FC
|
| 56-0454 |
Alexa Fluor® 700 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 104 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.
Applications Tested
The 104 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Shen, F-W. (1981). "Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. In Monoclonal antibodies and T cell Hybridomas; Perspective and technical advances, eds. Hammerling, G.J., U. Hammerling, and J.F. Kearney": 25-31.
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Mouse Cellular Antigens Chart