Product Information

Contents: Biotin anti-rat CD43
Catalog Number: 13-0430
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: HIS17
Isotype: Mouse IgG1, κ

Staining of Lou rat splenocytes with FITC anti-rat CD45R (HIS24) (cat. 11-0460) and .25 μg of Biotin Mouse IgG1, K Iso Cntrl (cat. 13-4714) (left) or .25 μg of Biotin anti-rat CD43 (HIS17) (right) followed by Streptavidin-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0430	Affinity Purified anti-rat CD43	N/A	N/A	FC IH/F
13-0430	Biotin anti-rat CD43	N/A	N/A	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HIS17 monoclonal antibody reacts with rat CD43, also known as leukosialin. CD43 is expressed on most mature T cells, NK cells, myeloid cells and plasma cells, but not resting B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIS17 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HIS17 antibody has been tested by flow cytometric analysis of rat splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kroese FG, Wubbena AS, Kuijpers KC, Nieuwenhuis P. 1987. The ontogeny of germinal centre forming capacity of neonatal rat spleen. Immunology. 60(4):597-602.
Kroese FG, Wubbena AS, Joling P, Nieuwenhuis P. 1985. T-lymphocytes in rat lymphoid follicles are a subset of T helper cells. Adv Exp Med Biol. 186:443-9.

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4714  Biotin Mouse IgG1, K Isotype Control