Also known as: fibrinogen receptor, gpIIb/IIIa, integrin alpha IIb, ITGA2b
RUO: For Research Use Only
Staining of mouse platelets with Anti-Mouse/Rat CD61 (Integrin β3) FITC (cat. 11-0611) and 0.06 µg of Rat IgG1 κ Isotype Control Biotin (cat. 13-4301) (left) or 0.06 µg of Anti-Mouse CD41 Biotin (right) followed by Streptavidin PE (cat. 12-4317).
Contents: Anti-Mouse CD41 Biotin Catalog Number: 13-0411 Concentration: 0.5 mg/ml Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Clone: eBioMWReg30 (MWReg30) Host/Isotype: Rat IgG1, κ
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Cat. No.
Size
Price
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13-0411-81
50 ug
13-0411-82
100 ug
13-0411-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The eBioMWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin αIIb). While initially thought to be expressed exclusively on the surface of platelets and megakaryocytes, it has been demonstrated that CD41 is also expressed on hematopoietic progenitors in the embryo, fetus and adult. CD41 associates with CD61 (gpIIIa, integrin βIII) to form a receptor which plays a major role in platelet function, including binding of several adhesion molecules such as fibrinogen, fibronectin and vitronectin.
Recently, the SLAM-family markers, CD48 and CD150 have been used to reliably identify hematopoietic stem cells (HSC). Specifically, it was found that CD150+CD48- bone marrow cells were highly efficient in their ability to confer long-term multi-lineage reconstitution in irradiated mice. Furthermore, the efficiency of reconstitution was enhanced when HSCs were further enriched through the exclusion of CD41+ cells. Thus, the use of CD150+CD48-CD41- as an expression profile efficiently identifies hematopoietic stem cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioMWReg30 (MWReg30) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioMWReg30 (MWReg30) antibody has been tested by flow cytometric analysis of mouse platelets. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Nieswandt B, Echtenacher B, Wachs FP, Schroder J, Gessner JE, Schmidt RE, Grau GE, Mannel DN.
Acute systemic reaction and lung alterations induced by an antiplatelet integrin gpIIb/IIIa antibody in mice. Blood. 1999 Jul 15;94(2):684-93. (MWReg30, FC, FA, IHC, IP, PubMed)
Teeling JL, Jansen-Hendriks T, Kuijpers TW, de Haas M, van de Winkel JG, Hack CE, Bleeker WK.
Therapeutic efficacy of intravenous immunoglobulin preparations depends on the immunoglobulin G dimers: studies in experimental immune thrombocytopenia.
Blood. 2001 Aug 15;98(4):1095-9. (MWReg30, FA, PubMed)
Mitjavila-Garcia MT, Cailleret M, Godin I, Nogueira MM, Cohen-Solal K, Schiavon V, Lecluse Y, Le Pesteur F, Lagrue AH, Vainchenker W.
Expression of CD41 on hematopoietic progenitors derived from embryonic hematopoietic cells.
Development. 2002 Apr;129(8):2003-13. (MWReg30, FC, PubMed)
Bertrand JY, Giroux S, Golub R, Klaine M, Jalil A, Boucontet L, Godin I, Cumano A.
Characterization of purified intraembryonic hematopoietic stem cells as a tool to define their site of origin.
Proc Natl Acad Sci U S A. 2005 Jan 4;102(1):134-9. (MWReg30, FC, PubMed)
