Product Information

Contents: Biotin anti-mouse CD40
Catalog Number: 13-0402
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: HM40-3
Isotype: Armenian Hamster IgM, κ

Staining of BALB/c splenocytes with 0.25 μg Biotin Golden Syrian Hamster IgG Iso Cntrl (cat. 13-4913) (open histogram) or 0.25 μg Biotin HM40-3 (colored histogram) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0402	Affinity Purified anti-mouse CD40	N/A	N/A	FA FC IHC
16-0402	Functional Grade* Purified anti-mouse CD40	N/A	N/A	FA FC
13-0402	Biotin anti-mouse CD40	N/A	N/A	FC
11-0402	FITC anti-mouse CD40	488	518	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HM40-3 monoclonal antibody reacts with mouse CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. HM40-3 has been reported in the literature to cross-react with the rat CD40 antigen.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HM40-3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HM40-3 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kashiwada, M., Y. Yagita, et al. 1996. Activation of mitogen-activated protein kinases via CD40 is distinct from that stimulated by surface IgM on B cells. Eur. J. Immunol. 26: 1451-58
Inaba, K., M. Witmer-Pack, et al. 1994. The tissue distrbution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. J. Exp. Med. 180:1849-60.
Kawano, T., J. Cui, et al. 1997. CD1d-restricted and TCR-mediated activation of Va14 NKT cells by glycosylceramides. Science 278: 1626-9
Ferlin, W. G., E. Severinson, et al. 1996. CD40 signaling induces interleukin-4-independent IgE switching in vivo. Eur J Immunol 26(12): 2911-5.
Trinite, B., C. Voisine, et al. 2000. A subset of cytolytic dendritic cells in rat. J. Immunol. 165(8): 4202-8.

Related Products

Cat. 12-0401  PE anti-mouse CD40 (clone 1C10)
Cat. 13-0401  Biotin anti-mouse CD40 (clone 1C10)
Cat. 14-0401  Affinity Purified anti-mouse CD40 (clone 1C10)
Cat. 15-0401  PE-Cy5 anti-mouse CD40 (clone 1C10)
Cat. 16-0401  Functional Grade Purified anti-mouse CD40 (clone 1C10)
Cat. 17-0401  APC anti-mouse CD40 (clone 1C10)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)