Biotin anti-human CD38 antibody: biotin conjugate

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Contents: Biotin anti-human CD38
Catalog Number: 13-0389
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: HIT2
Isotype: Mouse IgG1, κ
HLDA No.: III 155

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
13-0389-80	25 ug	$80
13-0389-82	100 ug	$180

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with 0.25 μg of Biotin Mouse IgG1, K Iso Cntrl (cat. 13-4714) (open histogram) or Biotin anti-human CD38 (HIT2) (colored histogram) followed by SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0389	FITC anti-human CD38	488	518	FC
12-0389	PE anti-human CD38	488	575	FC
13-0389	Biotin anti-human CD38	N/A	N/A	FC
14-0389	Affinity Purified anti-human CD38	N/A	N/A	FC IHC
15-0389	PE-Cy5 anti-human CD38	488	670	FC
17-0389	Allophycocyanin (APC) anti-human CD38	633	660	FC
25-0389	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD38	488	760	FC
30-0389	DISCONTINUED - Allophycocyanin-Cy5.5 (APC-Cy5.5) anti-human CD38 (Alexa Fluor® 700 Equivalent)	633	690	FC
56-0389	Alexa Fluor® 700 anti-human CD38	633	723	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HIT2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HIT2 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Joshua, D., A. Petersen, et al. (1996). "The labelling index of primitive plasma cells determines the clinical behaviour of patients with myelomatosis." Br J Haematol 94(1): 76-81.
Horenstein, A. L., H. Stockinger, et al. (1998). "CD38 binding to human myeloid cells is mediated by mouse and human CD31." Biochem J 330(Pt 3): 1129-35.
Deaglio, S., M. Morra, et al. (1998). "Human CD38 (ADP-ribosyl cyclase) is a counter-receptor of CD31, an Ig superfamily member." J Immunol 160(1): 395-402.

Related Products

Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4714    Biotin Mouse IgG1, K Isotype Control