Product Information

Contents: Biotin anti-mouse CD38
Catalog Number: 13-0381
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 90
Isotype: Rat IgG2a, κ

Staining of BALB/c splenocytes with 0.06 μg Biotin Rat IgG2a Iso Cntrl (cat. 13-4321) (open histogram) or 0.06 μg Biotin anti-mouse CD38 (90) (colored histogram) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0381	Affinity Purified anti-mouse CD38	N/A	N/A	FA FC IHC
16-0381	Functional Grade* Purified anti-mouse CD38	N/A	N/A	FA FC
13-0381	Biotin anti-mouse CD38	N/A	N/A	FC
11-0381	FITC anti-mouse CD38	488	518	FC
12-0381	PE anti-mouse CD38	488	575	FC
15-0381	PE-Cy5 anti-mouse CD38	488	670	FC
17-0381	APC anti-mouse CD38	633	660	FC
56-0381	Alexa Fluor® 700 anti-mouse CD38 (APC-Cy5.5 Equivalent)	633	723	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 90 monoclonal antibody reacts with the mouse CD38 molecule, an ~42 kDa type II transmembrane protein. CD38 is expressed at increasingly higher levels on B cells at each stage of B-cell differentiation, and is then down-regulated on germinal center B cells and mature plasma cells. Its expression is reported on a subpopulation of thymocytes, mature T cells, and NK cells. Crosslinking of CD38 on the surface of mature, resting B cells induces B-cell proliferation, which is enhanced by co-signals such as IL-4 and LPS. CD38, a counter-receptor for CD31, is an ectoenzyme with cyclase and hydrolase enzymatic activity and is speculated to play a role in lymphocyte activation and differentiation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 90 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 90 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Oliver, A. M., J. C. Grimaldi, et al. (1999). "Independently ligating CD38 and Fc gammaRIIB relays a dominant negative signal to B cells." Hybridoma 18(2): 113-9.
Oliver, A. M., F. Martin, et al. (1997). "Marginal zone B cells exhibit unique activation, proliferative and immunoglobulin secretory responses." Eur J Immunol 27(9): 2366-74.
Oliver, A. M., F. Martin, et al. (1997). "Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells." J Immunol 158(3): 1108-15.

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4321  Biotin Rat IgG2a Isotype Control