Product Information

Contents: Biotin anti-mouse CD30
Catalog Number: 13-0301
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: mCD30.1
Isotype: Armenian Hamster IgG

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-0301	PE anti-mouse CD30	488	575	FC
13-0301	Biotin anti-mouse CD30	N/A	N/A	FC
14-0301	Affinity Purified anti-mouse CD30	N/A	N/A	FA FC
16-0301	Functional Grade* Purified anti-mouse CD30	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The mCD30.1 monoclonal antibody reacts with mouse CD30, a 105-120 kDa member of the TNFR superfamily. CD30 is not detectable on splenocytes, but can be upregulated on T cells stimulated in vitro through TCR with a peak expression around day 4-5 post-activation. CD30 is expressed by both CD8⁺ and CD4⁺ activated cells. It is suggested that CD30 regulates cytokine secretion by T cells and has a role in T cell development.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The mCD30.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The mCD30.1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Bowen, M. A., R. K. Lee, et al. (1996). "Structure and expression of murine CD30 and its role in cytokine production." J Immunol 156(2): 442-9.

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