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Product Information


Contents: Biotin anti-human CD25 (Interleukin-2 Receptor, IL-2R)
Catalog Number: 13-0259
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: BC96
Isotype: Mouse IgG1, κ
HLDA No.: V T-072
 
 

 

Available Formats of This Product
Cat. No. Format Excite
(nm)
Emit
(nm)
Reported Applications
11-0259 **May be discontinued; see superior product Alexa Fluor® 488** FITC anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) 488 518 FC 
12-0259 PE anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) 488 575 FC 
13-0259 Biotin anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) N/A N/A FC 
14-0259 Affinity Purified anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) N/A N/A FC 
15-0259 Phycoerythrin-Cy5 (PE-Cy5) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) 488 670 FC 
17-0259 Allophycocyanin (APC) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) 633 660 FC 
25-0259 Phycoerythrin-Cy7 (PE-Cy7) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) 488 760 FC 
45-0259 PerCP-Cy5.5 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) 488 690 FC 
53-0259 Alexa Fluor® 488 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) 488 519 FC 

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description


The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor β chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.


Applications Reported


For research use only, not for diagnostic or therapeutic use. This BC96 antibody has been reported for use in flow cytometric analysis.


Applications Tested


This BC96 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.


References



Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.


Related Products


Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4714    Biotin Mouse IgG1, K Isotype Control



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