Product Information

Contents: Biotin anti-human CD24 (Heat Stable Antigen)
Catalog Number: 13-0247
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: eBioSN3 (SN3 A5-2H10)
Isotype: Mouse IgG1
HLDA No.: IV

Staining of normal human peripheral blood cells with 0.125 μg of Biotin Mouse IgG1, K Iso Cntrl (cat. 13-4714) (open histogram) or 0.125 μg of Biotin anti-human CD24 (SN3) (colored histogram) followed by SAv-PE (cat. 12-4317). Cells in the large scatter population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0247	Affinity Purified anti-human CD24 (Heat Stable Antigen)	N/A	N/A	FC
13-0247	Biotin anti-human CD24 (Heat Stable Antigen)	N/A	N/A	FC
11-0247	Fluorescein isothiocyanate (FITC) anti-human CD24 (Heat Stable Antigen)	488	518	FC
12-0247	Phycoerythrin (PE) anti-human CD24 (Heat Stable Antigen)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The SN3 monoclonal antibody reacts with the human CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioSN3 (SN3 A5-2H10) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioSN3 (SN3 A5-2H10) antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

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