Product Information

Contents: Biotin anti-mouse CD23 (FcεRII)
Catalog Number: 13-0232
Sizes: 50 ug, 100 ug, 500 ug, 1 mg
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: B3B4
Isotype: Rat IgG2a, κ

Two-color surface staining of mouse splenocytes with anti-mouse CD23 (B3B4) FITC and anti-mouse CD45R/B220 (RA3-6B2) PE. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0232	Affinity Purified anti-mouse CD23 (FceRII)	N/A	N/A	FC IHC
13-0232	Biotin anti-mouse CD23 (FceRII)	N/A	N/A	FC
11-0232	FITC anti-mouse CD23 (FceRII)	488	518	FC
12-0232	PE anti-mouse CD23 (FceRII)	488	575	FC
25-0232	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD23 (FceRII)	488	760	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on resting conventional B cells, and its expression is modulated upon B-cell activation. B-1 cell lineage (CD5⁺ B cells) does not express CD23. Soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B-cell activation. CD21 is thought to bind to CD23.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The B3B4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The B3B4 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Rabin, E., Y. Z. Cong, et al. 1992. Loss of CD23 is a consequence of B-cell activation. Implications for the analysis of B-cell lineages. Ann N Y Acad Sci 651: 130-42.
Cong, Y. Z., E. Rabin, et al. 1991. Treatment of murine CD5- B cells with anti-Ig, but not LPS, induces surface CD5: two B-cell activation pathways. Int Immunol 3(5): 467-76.
Lee, W. T., M. Rao, et al. 1987. The murine lymphocyte receptor for IgE. IV. The mechanism of ligand-specific receptor upregulation on B cells. J Immunol 139(4): 1191-8.
Rao, M., W. T. Lee, et al. 1987. Characterization of a monoclonal antibody directed against the murine B lymphocyte receptor for IgE. J Immunol 138(6): 1845-51.
Waldschmidt, T. J., F. G. Kroese, et al. 1991. The expression of B cell surface receptors. III. The murine low-affinity IgE Fc receptor is not expressed on Ly 1 or 'Ly 1-like' B cells. Int Immunol 3(4): 305-15.
Waldschmidt, T. J., D. H. Conrad, et al. 1988. The expression of B cell surface receptors. I. The ontogeny and distribution of the murine B cell IgE Fc receptor. J Immunol 140(7): 2148-54.

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