Product Information

Contents: Biotin anti-mouse CD22
Catalog Number: 13-0221
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 2D6
Isotype: Rat IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0221	Affinity Purified anti-mouse CD22	N/A	N/A	FC
13-0221	Biotin anti-mouse CD22	N/A	N/A	FC
11-0221	Fluorescein isothiocyanate (FITC) anti-mouse CD22	488	518	FC
12-0221	Phycoerythrin (PE) anti-mouse CD22	488	575	FC
15-0221	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD22	488	670	FC
17-0221	Allophycocyanin (APC) anti-mouse CD22	633	660	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 2D6 monoclonal antibody reacts with mouse CD22, which is a member of the Siglec (sialic acid-binding immunoglobulin-like lectin) family. CD22 is a 150 kDa type I membrane protein expressed by the B-cell lineage in a developmentally-regulated pattern. In mouse, CD22 is expressed at low levels on the surface of pre-B cells and immature (B220^loIgM^hi) B cells, but high levels on mature (B220^hiIgM^intIgD⁺) B cells in the bone marrow. In the periphery, mouse CD22 is highly expressed on all B-cell subsets. Furthermore, mouse CD22 expression is up-regulated slightly upon activation, however expression is lost upon differentiation to a plasma cell. CD22 plays a role in the negative regulation of B-cell Receptor (BCR) signaling, however it has also been demonstrated to function as an intercellular adhesion molecule through binding of sialic acid-bearing proteins expressed by lymphocytes, neutrophils, monocytes and non-hematopoietic cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 2D6 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 2D6 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Symington FW, Subbarao B, Mosier DE, Sprent J. Lyb-8.2: A new B cell antigen defined and characterized with a monoclonal antibody. Immunogenetics. 1982;16(5):381-91.

Torres RM, Law CL, Santos-Argumedo L, Kirkham PA, Grabstein K, Parkhouse RM, Clark EA. Identification and characterization of the murine homologue of CD22, a B lymphocyte-restricted adhesion molecule. J Immunol. 1992 Oct 15;149(8):2641-9.

Doody GM, Justement LB, Delibrias CC, Matthews RJ, Lin J, Thomas ML, Fearon DT. A role in B cell activation for CD22 and the protein tyrosine phosphatase SHP. Science. 1995 Jul 14;269(5221):242-4.

Related Products

Cat. 13-4301  Biotin Rat IgG1 Isotype Control
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)