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Product Information


Contents: Biotin anti-human CD20
Catalog Number: 13-0209
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 2H7
Isotype: Mouse IgG2b, κ
HLDA No.: IV B201
 
 
data image 1 data image 2
Staining of normal human peripheral blood cells with APC anti-human CD19 (HIB19) (cat. 17-0199) and 0.125 μg of Biotin Mouse IgG2b Iso Cntrl (cat. 13-4732) (left) or 0.125 μg of Biotin anti-human CD20 (2H7) (right). Cells in the lymphocyte gate were used for analysis. 

Available Formats of This Product
Cat. No. Format Excite
(nm)
Emit
(nm)
Reported Applications
14-0209 Affinity Purified anti-human CD20 N/A N/A FC  IH/F 
13-0209 Biotin anti-human CD20 N/A N/A FC  IH/F 
11-0209 FITC anti-human CD20 488 518 FC 
12-0209 PE anti-human CD20 488 575 FC 
15-0209 PE-Cy5 anti-human CD20 488 670 FC 
17-0209 Allophycocyanin (APC) anti-human CD20 633 660 FC 
25-0209 Phycoerythrin-Cy7 (PE-Cy7) anti-human CD20 488 760 FC 
56-0209 Alexa Fluor® 700 anti-human CD20 (APC-Cy5.5 Equivalent) 633 723 FC 
45-0209 PerCP-Cy5.5 anti-human CD20 (PE-Cy5.5 replacement) 488 690 FC 
94-0209 eFluorTM 625NC anti-human CD20 350-500 625 FC 
95-0209 eFluorTM 650NC anti-human CD20 350-500 650 FC 
47-0209 Allophycocyanin-eFluorTM 780 anti-human CD20 (APC-Alexa Fluor® 750 replacement) 633 780 FC 
48-0209 eFluorTM 450 anti-human CD20 405 450 FC 
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description


The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.


Applications Reported


For research use only, not for diagnostic or therapeutic use. This 2H7 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.


Applications Tested


This 2H7 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.


References



Reinherz, E.L., et al. eds. 1985. Leukocyte Typing II (Vol. I, II, and III). Human Leukocyte Differentiation Antigens detected by Monoclonal Antibodies.
McMichael, A.J., P.C.L. Beverly, et al. eds. 1987. Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.


Related Products


Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4732  Biotin Mouse IgG2b Isotype Control



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