Product Information

Contents: Biotin anti-mouse CD19
Catalog Number: 13-0191
Sizes: 50 ug, 100 ug, 500 ug, 1 mg
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: MB19-1
Isotype: Mouse IgA, κ

Staining of C57Bl/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.5 μg of Biotin anti-mouse CD19 (MB19-1) (colored histogram) followed by Streptavidin-PE (cat. 12-4312). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0191	Affinity Purified anti-mouse CD19	N/A	N/A	FA FC IP
16-0191	Functional Grade* Purified anti-mouse CD19	N/A	N/A	FA FC IP
13-0191	Biotin anti-mouse CD19	N/A	N/A	FC
11-0191	FITC anti-mouse CD19	488	518	FC
12-0191	PE anti-mouse CD19	488	575	FC
15-0191	PE-Cy5 anti-mouse CD19	488	670	FC
17-0191	APC anti-mouse CD19	633	660	FC
36-0191	Functional Grade* Biotin anti-mouse CD19	N/A	N/A	FA FC IP
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MB19-1 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. Staining of B cells with MB19-1 and its conjugates is usually dimmer than the rat anti-mouse CD19 antibody, clone 6D5.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MB19-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The MB19-1 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Engel, P., L. J. Zhou, et al. (1995). "Abnormal B lymphocyte development, activation, and differentiation in mice that lack or overexpress the CD19 signal transduction molecule." Immunity 3(1): 39-50.
Sato, S., N. Ono, et al. (1996). "CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity." J Immunol 157(10): 4371-8.
Sato, S., D. A. Steeber, et al. (1997). "CD19 expression levels regulate B lymphocyte development: human CD19 restores normal function in mice lacking endogenous CD19." J Immunol 158(10): 4662-9.
Tedder, T. F., M. Inaoki, et al. (1997). "The CD19-CD21 complex regulates signal transduction thresholds governing humoral immunity and autoimmunity." Immunity 6(2): 107-18.

Related Products

Cat. 12-0192  PE anti-mouse CD19 (clone 6D5)
Cat. 14-0192  **May be discontinued; Our scientists recommend clone eBio1D3, 14-0193 as an alternative** Affinity Purified anti-mouse CD19 (clone 6D5)
Cat. 15-0192  PE-Cy5 anti-mouse CD19 (clone 6D5)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)