Product Information

Contents: Biotin anti-mouse CD16/32 - blocks Fc binding
Catalog Number: 13-0161
Sizes: 50 ug, 100 ug, 500 ug, 1 mg
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 93
Isotype: Rat IgG2a, λ

Surface staining of mouse splenocytes with anti-mouse CD16/32 (93) APC (left), and PE-Cy7 (right). Autofluorescence is shown via open histogram. Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0161	Affinity Purified anti-mouse CD16/32 - blocks Fc binding	N/A	N/A	FA FC
16-0161	Functional Grade* Purified anti-mouse CD16/32 - blocks Fc binding	N/A	N/A	FA FC
13-0161	Biotin anti-mouse CD16/32 - blocks Fc binding	N/A	N/A	FC
11-0161	FITC anti-mouse CD16/32 - blocks Fc binding	488	518	FC
12-0161	PE anti-mouse CD16/32 - blocks Fc binding	488	575	FC
17-0161	APC anti-mouse CD16/32 - blocks Fc binding	633	660	FC
25-0161	PE-Cy7 anti-mouse CD16/32 - blocks Fc binding	488	760	FC
35-0161	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD16/32 - blocks Fc binding	488	690	FC
56-0161	Alexa Fluor® 700 anti-mouse CD16/32 - blocks Fc binding (APC-Cy5.5 Equivalent)	633	723	FC
45-0161	PerCP-Cy5.5 anti-mouse CD16/32 - blocks Fc binding (PE-Cy5.5 replacement)	488	690	FC
48-0161	eFluor^TM 450 anti-mouse CD16/32 - blocks Fc binding (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fcγ III Receptor) and CD32 (Fcγ II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 93 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 93 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Oliver, A. M., J. C. Grimaldi, et al. (1999). "Independently ligating CD38 and Fc gammaRIIB relays a dominant negative signal to B cells." Hybridoma 18(2): 113-9.

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