Product Information

Contents: Biotin anti-human CD15
Catalog Number: 13-0159
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: HI98
Isotype: Mouse IgM, κ
HLDA No.: IV M141

Surface staining of normal human peripheral blood cells with anti-human CD15 (HI98) FITC. Autofluorescence is indicated by open histogram. Cells in the granulocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0159	Affinity Purified anti-human CD15	N/A	N/A	FC IH/F
13-0159	Biotin anti-human CD15	N/A	N/A	FC IH/F
11-0159	FITC anti-human CD15	488	518	FC
57-0159	Pacific Blue® anti-human CD15	402	421	FC
94-0159	eFluor^TM 625^NC anti-human CD15	350-500	625	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HI98 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested

The HI98 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.06 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest

References

Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

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