Contents: Biotin anti-human CD15
Catalog Number: 13-0159
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: HI98
Isotype: Mouse IgM, κ
HLDA No.: IV M141
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Surface staining of normal human peripheral blood cells with anti-human CD15 (HI98) FITC. Autofluorescence is indicated by open histogram. Cells in the granulocyte population were used for analysis.
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Description
The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The HI98 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.
Applications Tested
The HI98 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.06 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
References
Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
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Human Cellular Antigens Chart