Product Information

Contents: Biotin anti-human mature macrophage marker (late-stage)
Catalog Number: 13-0115
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: eBio25F9 (25F9)
Isotype: Mouse IgG1

Staining of cultured macrophages from peripheral blood monocytes with 0.125 μg of Biotin Mouse IgG1, K Iso Cntrl (cat. 13-4714) (blue histogram) or 0.125 μg of Biotin anti-human mature macrophage marker (25F9) (purple histogram) followed by Streptavidin-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0115	Affinity Purified anti-human mature macrophage marker (late-stage)	N/A	N/A	FC IH/F IHC(Paraffin) IP WB
13-0115	Biotin anti-human mature macrophage marker (late-stage)	N/A	N/A	FC
51-0115	Alexa Fluor® 647 anti-human mature macrophage marker (late-stage)	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody 25F9 recognizes a protein on mature macrophages both on the cell surface and in intracellular vesicular structures. Expression is thought to correlate with endocytic macrophages and is useful in many disease models. Expression is not found on immature macrophages or monocytes or any other cell hematopoietic cell.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio25F9 (25F9) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio25F9 (25F9) antibody has been tested by flow cytometric analysis of cultured human macrophages. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4714  Biotin Mouse IgG1, K Isotype Control