Contents: Biotin anti-human mature macrophage marker (late-stage)
Catalog Number: 13-0115
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: eBio25F9 (25F9)
Isotype: Mouse IgG1
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Staining of cultured macrophages from peripheral blood monocytes with 0.125 μg of Biotin Mouse IgG1, K Iso Cntrl (cat. 13-4714) (blue histogram) or 0.125 μg of Biotin anti-human mature macrophage marker (25F9) (purple histogram) followed by Streptavidin-PE (cat. 12-4317). Total viable cells were used for analysis.
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Description
The monoclonal antibody 25F9 recognizes a protein on mature macrophages both on the cell surface and in intracellular vesicular structures. Expression is thought to correlate with endocytic macrophages and is useful in many disease models. Expression is not found on immature macrophages or monocytes or any other cell hematopoietic cell.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio25F9 (25F9) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio25F9 (25F9) antibody has been tested by flow cytometric analysis of cultured human macrophages. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Related Products
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11-4317 Streptavidin-FITC (Fluorescein isothiocyanate)
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12-4317 Streptavidin-PE (Phycoerythrin)
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17-4317 Streptavidin Allophycocyanin (SA-APC)
Cat.
13-4714 Biotin Mouse IgG1, K Isotype Control
Human Cellular Antigens Chart