Product Information

Contents: Biotin Human CD10
Catalog Number: 13-0108
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: eBioSN5c (SN5c L4-1A1)
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with 0.125 μg of Biotin Mouse IgG1, K Iso Cntrl (cat. 13-4714) (open histogram) or 0.125 μg of Biotin Human CD10 (colored histogram) followed by SAv-PE (cat. 12-4317). Cells in the granulocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0108	Affinity Purified anti-human CD10 (CALLA)	N/A	N/A	FC IH/F IP WB
13-0108	Biotin anti-human CD10 (CALLA)	N/A	N/A	FC IH/F
11-0108	Fluorescein isothiocyanate (FITC) anti-human CD10 (CALLA)	488	518	FC
12-0108	Phycoerythrin (PE) anti-human CD10 (CALLA)	488	575	FC
95-0108	eFluor™ 650^NC anti-human CD10 (CALLA)	350-500	650	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioSN5c monoclonal antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), which is a 100 kDa, type II cell surface glycoprotein originally identified for its expression on most acute lymphoblastic leukemias (ALLs). Subsequently, CD10 was shown to be the same molecule as neutral endopeptidase (NEP), or KII-NA. CD10 is a Zn²⁺-dependent metallopeptidase acting on substrates such as endothelin, glucagon, gastrin, neurotensin and bradykinin. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils. In B cells, CD10 regulates stromal cell-dependent B lymphopoiesis and its expression has been reported on mature germinal center B cells. CD10 expression is also found on normal donor granulocytes and bone marrow stromal cells. Most likely, CB-CALLA and eBioSN5c see different epitopes due to their ability to co-stain.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioSN5c (SN5c L4-1A1) antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.

Applications Tested

This eBioSN5c (SN5c L4-1A1) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Biddle WC, Haruta Y, Seon BK, Henderson ES, Sarcione EJ. In vitro and in vivo cytotoxic activity of anti-human leukemia monoclonal antibodies SN5c and SN6 daunorubicin conjugates. Leuk Res. 1989;13(8):699-707.

Matsuzaki H, Haruta Y, Fukukawa T, Barcos MP, Seon BK. Unique epitopes of common acute lymphoblastic leukemia antigen detected by new monoclonal antibodies. Cancer Res. 1987 Apr 15;47(8):2160-6. (PubMed)

Related Products

Cat. 13-0106 Biotin anti-human CD10 (CALLA, NEP, enkephalinase, Neprilysin) (clone eBioCB-CALLA (CB-CALLA))
Cat. 13-4714 Biotin Mouse IgG1, K Isotype Control