Product Information

Contents: Biotin anti-human CD9
Catalog Number: 13-0098
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: eBioSN4 (SN4 C3-3A2)
Isotype: Mouse IgG1

Staining of normal human platelets with 0.25 μg of Biotin Mouse IgG1, K Iso Cntrl (cat. 13-4714) (open histogram) or 0.25\ug Biotin anti-human CD9 (SN4) (colored histogram) followed by PE-SAV (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0098	Affinity Purified anti-human CD9	N/A	N/A	FC IH/F IP
13-0098	Biotin anti-human CD9	N/A	N/A	FC
11-0098	Fluorescein isothiocyanate (FITC) anti-human CD9	488	518	FC
12-0098	Phycoerythrin (PE) anti-human CD9	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioSN4 monoclonal antibody reacts with human CD9. CD9 is a 24 kDa member of the tetraspanin family, whose members are characterized by the presence of 4 hydrophobic transmembrane domains. CD9 is expressed in platelets, eosinophils, basophils, pre-B cells, activated T cells and neural cell lines. Furthermore, CD9 expression has been associated with a malignant phenotype, including expression on 90% of non T cell acute lymphoblastic leukemia cells and on 50% of chronic lymphocytic and acute myeloblastic leukemias. In platelets, CD9 is expressed in α-granules and through association with the integrin αIIb/βIII plays a role in platelet aggregation, as demonstrated by the use of anti-human CD9 antibodies. CD9 has also been shown to induce the aggregation of pre-B cell lines, and the adhesion and migration of pre-B cells and Schwann cells. Additionally, it has been demonstrated that CD9 is able to provide a co-stimulatory signal for T cells independently of CD28, in the absence of antigen-presenting cells. Binding of the eBioSN4 monoclonal antibody partially cross-blocks binding of another anti-human CD9 monoclonal antibody, MM2/57.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioSN4 (SN4 C3-3A2) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioSN4 (SN4 C3-3A2) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Luo Y, Hara H, Haruta Y, Seon BK. Establishment of ascitic tumor of human pre-B acute lymphoblastic leukemia in nonconditioned nude mice. Cancer Res. 1989 Feb 1;49(3):706-10. (SN4, PubMed)

Ito T, Inaba M, Inaba K, Toki J, Sogo S, Iguchi T, Adachi Y, Yamaguchi K, Amakawa R, Valladeau J, Saeland S, Fukuhara S, Ikehara S. A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells. J Immunol. 1999 Aug 1;163(3):1409-19.

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4714  Biotin Mouse IgG1, K Isotype Control