Product Information

Contents: Biotin anti-human CD8a
Catalog Number: 13-0089
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: HIT8a
Isotype: Mouse IgG1, κ
HLDA No.: V 5T-CD08.10

Profile of biotin labeled mAb HIT8a (CD8) staining on human peripheral blood lymphocytes and detection with Streptavidin-PE (cat.no. 12-4317)

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0089	Affinity Purified anti-human CD8 (CD8a; alpha chain)	N/A	N/A	FC IH/F
16-0089	Functional Grade* Purified anti-human CD8 (CD8a; alpha chain)	N/A	N/A	FC
13-0089	Biotin anti-human CD8 (CD8a; alpha chain)	N/A	N/A	FC
11-0089	FITC anti-human CD8 (CD8a; alpha chain)	488	518	FC
12-0089	PE anti-human CD8 (CD8a; alpha chain)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HIT8a monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a . CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIT8a antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HIT8a antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

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