Product Information

Contents: Biotin Anti-human CD7
Catalog Number: 13-0079
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: eBio124-1D1 (124-1D1)
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with APC anti-human CD3 (UCHT1) (cat. 17-0038) and 0.5 μg of Biotin Mouse IgG1, K Iso Cntrl (cat. 13-4714) (left) or Biotin Anti-human CD7 (124-1D1) (right) followed by SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-0079	Phycoerythrin (PE) anti-human CD7	488	575	FC
13-0079	Biotin anti-human CD7	N/A	N/A	FC
14-0079	Affinity Purified anti-human CD7	N/A	N/A	FC
15-0079	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD7	488	670	FC
17-0079	Allophycocyanin (APC) anti-human CD7	633	660	FC
56-0079	Alexa Fluor® 700 anti-human CD7	633	723	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio124-1D1 monoclonal antibody reacts with human CD7, also known as gp40 and Leu9. CD7, a 40 kD receptor, is a member of the immunoglobulin gene superfamily. The N-terminal amino acid sequence (aa1-107) is highly homologous to Ig kappa light chain sequence; while the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T lymphocytes, and T cell leukemias. It is also found on natural killer cells, a small suppopulation of normal B cells and on maligant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity, as measured by calcium flux, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.

A clonogenic subpopulation of human CD34(+) CD38(-) cord blood cells that express CD45RA and HLA-DR and high levels of the CD7 has been reported. These cells possess the capacity for lymphopoiesis. They can generate B-cells, natural killer cells, and dendritic cells but do not possess the capacity to develop into myeloid cells or erythroid cells. The CD7(+) phenotype distinguishes primitive human lymphoid progenitors from pluripotent stem cells.

Furthermore, it has been suggested that CD7 co-operates with CD28 during Treg function, as mice deficient in both CD28 and CD7 have reduced total numbers of Tregs and these Tregs have reduced suppressive activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio124-1D1 (124-1D1) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio124-1D1 (124-1D1) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1.0 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sato AI, Balamuth FB, Ugen KE, Williams WV, Weiner DB. Identification of CD7 glycoprotein as an accessory molecule in HIV-1-mediated syncytium formation and cellfree infection. J Immunol. 1994 May 15;152(10):5142-52. (124-1D1, FA, FC, PubMed)

Aruffo A, Seed B. Molecular cloning of two CD7 (T-cell leukemia antigen) cDNAs by a COS cell expression system. EMBO J. 1987 Nov;6(11):3313-6.

Lyman SD, Escobar S, Rousseau AM, Armstrong A, Fanslow WC. Identification of CD7 as a cognate of the human K12 (SECTM1) protein. J Biol Chem. 2000 Feb 4;275(5):3431-7.

Related Products

Cat. 13-0077    Biotin anti-human CD7 (clone CD7-6B7)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4714    Biotin Mouse IgG1, K Isotype Control