Product Information

Contents: Biotin anti-human CD4
Catalog Number: 13-0048
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: OKT4 (OKT-4)
Isotype: Mouse IgG2b

Staining of normal human peripheral blood cells with 0.06 μg of Biotin Mouse IgG2b Iso Cntrl (cat. 13-4732) (open histogram) or 0.06 μg of Biotin anti-human CD4 (OKT4) (colored histogram) followed by Streptavidin-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0048	Fluorescein isothiocyanate (FITC) anti-human CD4	488	518	FC
12-0048	Phycoerythrin (PE) anti-human CD4	488	575	FC
13-0048	Biotin anti-human CD4	N/A	N/A	FC
14-0048	Affinity Purified anti-human CD4	N/A	N/A	FC IP
16-0048	Functional Grade* Purified anti-human CD4	N/A	N/A	FC IP
17-0048	Allophycocyanin (APC) anti-human CD4	633	660	FC
35-0048	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-human CD4	488	690	FC
45-0048	PerCP-Cy5.5 anti-human CD4	488	690	FC
51-0048	Alexa Fluor® 647 anti-human CD4	633	668	FC
53-0048	Alexa Fluor® 488 anti-human CD4	488	519	FC
56-0048	Alexa Fluor® 700 anti-human CD4 (APC-Cy5.5 Equivalent)	633	723	FC
57-0048	Pacific Blue® anti-human CD4	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This OKT4 (OKT-4) antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Reinherz EL, Kung PC, Goldstein G, Schlossman SF. 1979. Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci. 76(8): 4061-5.

Bour S, Boulerice F, Wainberg MA. Inhibition of gp160 and CD4 maturation in U937 cells after both defective and productive infections by human immunodeficiency virus type 1. J Virol. 1991 Dec;65(12):6387-96. (OKT4, IP, PubMed)

Related Products

Cat. 11-0049    FITC anti-human CD4 (clone RPA-T4)
Cat. 12-0049    PE anti-human CD4 (clone RPA-T4)
Cat. 13-0049    Biotin anti-human CD4 (clone RPA-T4)
Cat. 14-0049    Affinity Purified anti-human CD4 (clone RPA-T4)
Cat. 15-0049    PE-Cy5 anti-human CD4 (clone RPA-T4)
Cat. 16-0049    Functional Grade Purified anti-human CD4 (clone RPA-T4)
Cat. 17-0049    APC anti-human CD4 (clone RPA-T4)
Cat. 25-0049    PE-Cy7 anti-human CD4 (clone RPA-T4)
Cat. 27-0049    Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD4 (APC-Cy7 Equivalent) (clone RPA-T4)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4732    Biotin Mouse IgG2b Isotype Control